It is critical to follow the right autoclave sterilisation processes. The system fails if protocols are not observed at all times, and everyone’s health and safety are jeopardised. Autoclaves are used to sterilise various items, not simply medical equipment, as you may believe. Here’s everything you need to know about autoclave sterilisation, including how it works, how to do it correctly, and the implications of mistakes produced by malfunctioning equipment.

Sterilisation process

To avoid the entry of organisms into the sterile location, professionals should use stringent sterile procedures when creating clean equipment. All invasive dental operations require strict sterile techniques.



Handwashing with antimicrobial soap before sterile procedures is the most basic clean approach. Healthcare personnel should wash their hands between each sanitary operation and after interaction with nonsterile patients or equipment.

The Centers for Disease Control and Prevention (CDC) recommends using sterile procedures to avoid infection. This is an essential aspect of medical treatment that must be followed precisely to guarantee patient safety. When caring for a patient, hygiene should always come first. All microorganisms, including bacteria, viruses, and fungus, are absent from a sterile field.

Environmental Disinfection

Maintaining a sterile field at all times is one of the most powerful and effective techniques to avoid infection. A sterile area can be created and maintained in a variety of ways. Using uncompromising process standards is the best approach to achieve this. When setting up sterile equipment, the disinfection and sanitation process guidelines contain a checklist.

Use of Sterilization Equipment

As dental health professionals, it is a part of their obligation to provide patients with the highest quality of care. Following the standard practices is crucial to successfully performing our duties and responsibilities, like using appropriate personal protective equipment and sterile instruments. By doing so, you prevent the spread of diseases and keep your patients safe. One of the essential requirements to maintain a secure and healthy environment for dental patients is sterilisation equipment. These machines are vital for properly cleaning and disinfection of our dental devices and accessories.

Ultrasonic Cleaner

dental sterilisation cycleIt would be best if you rinsed the instruments under running water after each use to remove any particles or saliva that may have been collected. After the tools have been rinsed, they should be placed in a cassette, which is a plastic tray with drainage holes. This is the ultrasonic cleaner.

The ultrasonic cleaner uses a cassette placed in a huge tube filled with water. In about 10 minutes, this technique eliminates even more trash and particles from the equipment. The ultrasonic cleaner has a timer, and it is vital to stick to it. If you leave the instruments in for too long, they may corrode.


One of the most essential devices that any healthcare professional should have is an autoclave steriliser. The purpose of this machine is to sanitise lab equipment and trash by killing microorganisms such as spores and bacteria using pressurised steam or heat.

Autoclave supplies are used in the dentistry business to sterilise dental burs, dental handpieces, and other dental equipment. They may also be employed in demanding sterilisation operations in safety laboratories, such as sterilising liquids, solids, wastes, and biological dangers.

The most critical aspect of sterilisation cycles is keeping instruments clean and avoiding infection and other ailments. In brief, having autoclaves in your office will assist you in keeping your services secure and healthy.

How to prepare for sterilisation

Tool cleaning and preparation

Cleaning and preparing the dental and surgical instruments for following operations is the first step in decontamination. Any tool that is used frequently handled roughly, or cleaned with abrasive materials, can be damaged or dulled. Instruments should be assessed regularly and replaced when necessary. Scissors and other sharp tools can sometimes be re-sharpened to prolong life.

As part of the instrument preparation, they must be gently cleaned after each use to ensure that all blood and tissue are removed. This is a critical action since even minute biological residues might obstruct the sterilising process. Keep an eye out for any difficult-to-reach grooves or nooks. Handwashing with soft brushes, sprinkling, rinsing, and ultrasonic cleaning in special washers are all options for this stage.


Disinfection is the next step in the tool sterilisation process. This can be thermal, chemical-thermal, or chemical disinfection, depending on the manufacturer’s recommendations. It can be done in a separate machine or in a washing machine for disinfection. When using a chemical disinfectant, special attention should be paid to the correct disinfectant concentration, in which the equipment should be totally submerged.


Dry the instruments thoroughly by hand (e.g. with paper towels) or with a dryer using dry, pressurised air before packaging them in special protective containers. If manually made, they should be dried as soon as possible to avoid microbe proliferation. After thoroughly drying the instruments, use a specifically developed agent to keep them in good condition.

packaging for sterilisationPackaging for tools

We must remember to pick the suitable sort of packaging for the sterilising process to go well. Packages or pouches should be stacked in the autoclave to ensure the highest sterilisation efficiency. This should follow the principle of foil to foil, paper to paper.

Loading the sterilisation chamber

There are a few basic rules to remember. First of all, heavy instruments should be placed at the bottom of the sterilisation chamber and lighter ones at higher levels. The minimum filling of the chamber should also not be less than 1/6 of its capacity. Please pay attention to the maximum acceptable load size and weight, as specified by the producer of the autoclave. Biological and chemical indicators are essential to ensure that the sterilisation processes run correctly. Chemical indicators are tests dedicated to the selected sterilisation method, and biological indicators are ampoule and strip tests containing specially developed, resistant strains of bacteria intended for incubation.


Only by following the manufacturer’s instructions will you be able to carry out an effective sterilisation process in an autoclave. This process should not damage or alter the properties of the sterilised instruments. Choose the settings that match the type of the tools you want to sterilise – if the equipment is smooth, without gouges, etc., a shorter program can be selected. Do not forget about distilled water necessary for the entire sterilisation process.

End of sterilisation

After the instrument sterilisation cycle is finished, remove the tools to let them cool down. Then, keeping them in their pouches, place them in the specific place where they should be stored. The packaging should include the date of sterilisation or the expiry date and a description of the contents, in the case of non-transparent packaging.

Why choose Melag Autoclave?

There is a wide range of autoclaves on the market, and it can be hard to choose which one is best for you and your dental practice. However, having a reliable autoclave, like Melag Autoclave, is undoubtedly one of the best decisions you will ever make for your profession. Here are some of the benefits you will enjoy when you choose from the Melag autoclaves that Melag Autoclave carries:

  • Original and innovative autoclave designs
  • It has a user-friendly interface.
  • Certain styles of Melag Autoclaves have an optional printer as well as Data Recording systems, making sterilisation documentation easy and hassle-free
  • It has high-performance vacuum and steam systems that will get your instruments to sterilise quickly.
  • Unlike some other autoclave options, Melag Vacuklav units have integrated water containers for easy disposal of wastewater.

So, if you are concerned about patient safety when it comes to your medical or dental business, you need an autoclave. Melag autoclave and sterilisers available at Melag Autoclave are among the best choices available in the market. Contact us at (02) 8880 7813 or buy them online through the Melag Autoclave website to make it easier for you to run your business safely and effectively.


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